Journal: Journal of Neuroinflammation

Article Title: Presence and activation of pro-inflammatory macrophages are associated with CRYAB expression in vitro and after peripheral nerve injury

doi: 10.1186/s12974-021-02108-z

Figure Lengend Snippet: Secretion of cytokines by pro-inflammatory LPS/IFN-γ and anti-inflammatory IL-4/IL-13 -polarized macrophages following treatment with CRYAB. a mRNA levels of iNOS, IL-12p40, arginase-1, and IL-10 in LPS/IFN-γ- and IL-4/IL-13-polarized macrophages; representative of 3 experiments, n =3–4 wells/group. b – i Concentration of IL-6 ( b , f ), IL-1β ( c , g ), IL-12p40 ( d , h ), and TNF-α ( e , i ) secreted by pro-inflammatory LPS/IFN-γ ( b – e ) and anti-inflammatory IL-4/IL-13 ( f – i ) -polarized macrophages from WT (white bars) and Cryab −/− (black bars) animals grown for 48 h in the presence or absence of a CRYAB peptide; representative of 3 experiments, n =4 wells/group. Data represent mean ± sem, * p < 0.05 independent t -test

Article Snippet: The tissues were incubated overnight at 4 °C with rabbit anti-Iba1 (Wako Chemicals, 019-1974, 1:200) or goat anti-Iba1 (Novus Biologicals, NB100-1028, 1:100), rat anti-mouse CD16/32 (BD Biosciences, 553842, 1:500), goat anti-mouse CD206 (Santa Cruz Biotechnologies, sc-34577, 1:500), chicken anti-P0 (Abcam, ab39375, 1:500), rabbit anti-CD68 (Cell Signaling, 97778, 1:100), rabbit anti-arginase 1 (Cell Signaling, 93668, 1:50), rabbit anti-iNOS (BD Transduction Laboratories, 1:100; gift from Dr. Samuel David, McGill University), and DAPI (Invitrogen, D3571, 1:2000).

Techniques: Concentration Assay